Journal: Molecular medicine reports

Article Title: Long non‑coding RNA SNHG14 affects the proliferation and apoptosis of childhood acute myeloid leukaemia cells by modulating the miR‑193b‑3p/MCL1 axis.

doi: 10.3892/mmr.2020.11729

Figure Lengend Snippet: Figure 1. SNHG14 gene expression is upregulated in bone marrow tissues of patients with AML and AML cell lines. (A) Relative expression of SNHG14 in 57 AML bone marrow tissues and NBM. (B) Relative expression of SNHG14 in AML cell lines and human normal bone marrow CD34+ cells. **P<0.01 vs. CD34+ cells. The 2‑ΔΔCq method was used to analyse the relative mRNA expression level using GAPDH as an internal control. AML, acute myeloid leukaemia; NBM, normal marrow tissues; SNHG14, small nucleolar RNA host gene 14.

Article Snippet: Human normal bone marrow CD34+ cells and AML cell lines (MV‐4‐11, AML‐193, HL‐60, and KG‐1 cells) were obtained from the American Type Culture Collection and cultured in Dulbecco's modified Eagle's medium (DMEM; HyClone; GE Healthcare) containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C.

Techniques: Gene Expression, Expressing, Control

Journal: Molecular medicine reports

Article Title: Long non‑coding RNA SNHG14 affects the proliferation and apoptosis of childhood acute myeloid leukaemia cells by modulating the miR‑193b‑3p/MCL1 axis.

doi: 10.3892/mmr.2020.11729

Figure Lengend Snippet: Figure 3. miR‑193b‑3p is a target of SNHG14 in AML cells. (A) Starbase was used to predict the binding site between SNHG14 and miR‑193b‑3p. (B) Relative expression of miR‑193b‑3p in MV‑4‑11 and AML‑193 cells following SNHG14 silencing. **P<0.01 vs. blank control. (C) The target association between SNHG14 and miR‑193b‑3p was determined using an RNA immunoprecipitation assay. **P<0.01 vs. Anti‑IgG. (D) The target association between SNHG14 and miR‑193b‑3p was determined using a dual luciferase reporter gene assay. **P<0.01 vs. miR‑NC. (E) Relative expression of miR‑193b‑3p in 57 AML bone marrow tissues and NBM was detected by RT‑qPCR. (F) Spearman's correlation analysis was performed to evaluate the correlation between SNHG14 and miR‑193b‑3p expression. (G) Relative expression of miR‑193b‑3p in AML cell lines and human normal bone marrow CD34+ cells. **P<0.01 vs. CD34+ cells. The 2‑ΔΔCq method was used to analyse the relative mRNA expression level using U6 as an internal control. SNHG14, small nucleolar RNA host gene 14; miR, microRNA; si, small interfering RNA; NC, negative control; wt, wildtype; mut, mutated; NBM, normal marrow tissues; AGO2, protein argonaute‑2; AML, acute myeloid leukaemia.

Article Snippet: Human normal bone marrow CD34+ cells and AML cell lines (MV‐4‐11, AML‐193, HL‐60, and KG‐1 cells) were obtained from the American Type Culture Collection and cultured in Dulbecco's modified Eagle's medium (DMEM; HyClone; GE Healthcare) containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C.

Techniques: Binding Assay, Expressing, Control, RNA Immunoprecipitation, Luciferase, Reporter Gene Assay, Small Interfering RNA, Negative Control

Journal: Molecular medicine reports

Article Title: Long non‑coding RNA SNHG14 affects the proliferation and apoptosis of childhood acute myeloid leukaemia cells by modulating the miR‑193b‑3p/MCL1 axis.

doi: 10.3892/mmr.2020.11729

Figure Lengend Snippet: Figure 5. miR‑193b‑3p targets MCL1 in AML cells. (A) TargetScan was used to predict the binding site between miR‑193b‑3p and MCL1. (B) A dual luciferase reporter gene assay was employed to verify the target association between miR‑193b‑3p and MCL1. **P<0.01 vs. miR‑NC. (C) Relative expression of MCL1 in 57 AML bone marrow tissues and NBM was detected by RT‑qPCR. (D) Spearman's correlation analysis was performed to evaluate the correlation between MCL1 and miR‑193b‑3p expression. (E) Spearman's correlation analysis was performed to evaluate the correlation between MCL1 and SNHG14 expression. (F) Relative expression of miR‑193b‑3p in AML cell lines and human normal bone marrow CD34+ cells. **P<0.01 vs. CD34+ cells. The 2‑ΔΔCq method was used to analyse the relative mRNA expression level using GAPDH as an internal control. SNHG14, small nucleolar RNA host gene 14; miR, microRNA; NC, negative control; wt, wildtype; mut, mutated; NBM, normal marrow tissues; AML, acute myeloid leukaemia; MCL1, MCL1 apoptosis regulator BCL2 family member.

Article Snippet: Human normal bone marrow CD34+ cells and AML cell lines (MV‐4‐11, AML‐193, HL‐60, and KG‐1 cells) were obtained from the American Type Culture Collection and cultured in Dulbecco's modified Eagle's medium (DMEM; HyClone; GE Healthcare) containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C.

Techniques: Binding Assay, Luciferase, Reporter Gene Assay, Expressing, Control, Negative Control

Journal: Cell communication and signaling : CCS

Article Title: O-GlcNAcylated FTO promotes m6A modification of SOX4 to enhance MDS/AML cell proliferation.

doi: 10.1186/s12964-025-02058-6

Figure Lengend Snippet: Fig. 2 O‑GlcNAcylation weakens FTO expression. (A) Volcano diagram representing FTO expression after OSMI-1 treatment in KG1a cells; p-val ue < 0.05,|Fold change| > 1.5. (B) The correlation between O-GlcNAc levels and the survival of AML patients was explored using upper quartile analysis through the Kaplan-Meier Plotter. (C) The association between O-GlcNAc and FTO in a cohort of 14 MDS/AML patients by ELISA. (D) Western blot detec tion of FTO level in cKit + cells of the bone marrow from MLL-AF9-induced leukemia mice. (E) FTO level in primary CD34 + cells from MDS/AML patients after 20 µM DMSO and 20 µg/mL OSMI-1 treatment. (F-G) FTO level in KG1a and SKM1 cells after TMG and OSMI-1 treatment

Article Snippet: CD34+ cells were treated with 20 μM DMSO and 20 μg/mL OSMI-1 (MCE, NJ, USA) for 48 h, respectively.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: The Journal of thoracic and cardiovascular surgery

Article Title: Endothelial preservation of the minimally manipulated saphenous vein composite graft: histologic and immunohistochemical study.

doi: 10.1016/j.jtcvs.2012.01.017

Figure Lengend Snippet: FIGURE 4. Luminal endothelium of the SV in the control (A), conven- tional (B), and composite groups (C). CD34 was stained as a brown color (A: 3200; B, C: 3100). Note the defects in staining (black arrows), partic- ularly in the conventional group.

Article Snippet: Immunohistochemistry was performed using mouse monoclonal antibodies against human endothelial glycoprotein CD31 (1:25, Santa Cruz Biotechnology, Inc, Santa Cruz, Calif), CD34 (1:10, Cell Signaling Technology, Inc, Boston, Mass), von Willebrand factor (vWF 1:200, Dako, DK-2600 Glostrup, Denmark), and endothelial nitric oxide synthase (eNOS 1:50, R&D Systems, Inc, Minneapolis, Minn).

Techniques: Control, Staining

Journal: JCI Insight

Article Title: Differential CXCR4 expression on hematopoietic progenitor cells versus stem cells directs homing and engraftment

doi: 10.1172/jci.insight.151847

Figure Lengend Snippet: ( A – D ) Irradiated NSG mice were transplanted with MPB CD34 + HSPC that were transduced with a GFP-encoding LV vector (gene transfer 63%–66%) either i.v. or i.b.m. at the indicated cell doses. Human cell engraftment was analyzed by determining the percentage of human CD45 + (hCD45 + ) cells and GM hCD45 + (hCD45 + GFP + ) cells in the BM obtained via BM aspirate at 12 weeks to assess short-term engraftment ( A and B ) and from pooled BM from femurs, tibias, and iliac crests at 24 weeks to determine long-term human engraftment ( C and D ). Bars represent median engraftment ± 95% CI. Each symbol represents an individual mouse; n = 18–20 mice per cell dose per transplant method. Mice were transplanted using 4 unique MPB donors in 4 experiments; statistical analysis was performed using 1-way ANOVA comparing i.v. and i.b.m. groups at each cell dose with correction for multiple comparisons. ( E – H ) Lineage output of the human xenograft. BM was stained with antibodies specific for T cell lineage (hCD3), B cell lineage (hCD19), myeloid lineage (hCD33), and HSPC (hCD34) to determine the lineage output of the transplanted total HSPC ( E and G ) and GM HSPC ( F and H ) at 12 weeks and 24 weeks after transplant. Statistical analysis was performed using 2-way ANOVA. * P < 0.05, ** P < 0.01.

Article Snippet: CD34 + CD38 – cells were isolated from G-CSF MPB by magnetic selection with human CD34 + CD38 – Isolation Kit (Miltenyi Biotec, 130-114-822) and cryopreserved.

Techniques: Irradiation, Transduction, Plasmid Preparation, Staining

Journal: JCI Insight

Article Title: Differential CXCR4 expression on hematopoietic progenitor cells versus stem cells directs homing and engraftment

doi: 10.1172/jci.insight.151847

Figure Lengend Snippet: ( A ) MPB CD34 + HSPC were injected i.v., i.b.m. using a standard syringe, or slow i.b.m. using a Hamilton syringe over 1 minute, and homing of CD34 + cells to the BM was analyzed 20–22 hours later. To determine if mechanical pressure in the injected femur alters homing, irradiated CD34 – cells were injected i.b.m., and CD34 + cells were injected i.v. into the same animal. Homing was determined in the injected femurs (IF) and non-IF with i.b.m. injections, and in both femurs (BF) combined with i.v. injection. Symbols represent individual animals; n = 3 mice per group; statistical analysis was performed using 2-way ANOVA. ( B and C ) For i.b.m. injections, BM from the IF and non-IF was analyzed for expression of CXCL12 and VCAM1 on mouse stromal cells (hCD45 – PKH-26 – , mouse lineage – mCD45 – mCD51 + cells). n = 5 mice per group; data were normalized to i.v. injected group; statistical analysis was performed by Mann-Whitney U test. ( D and E ) CXCR4 and VLA4 homing receptor expression of CD34 + HSPC, CD34 + CD38 + HPC, and CD34 + CD38 – CD90 + HSC-enriched populations. Data were normalized to CXCR4 and VLA4 expression on CD34 + HSPC. Symbols represent unique MPB donors; n = 5 per group; statistical analysis was performed using ANOVA. ( F ) Adhesion of CD34 + HSPC, CD34 + CD38 + HPC, and CD34 + CD38 – CD90 + HSC to CXCL12. Symbols represent unique MPB donors; n = 5 per group; statistical analysis was performed using ANOVA. ( G ) Binding of CD34 + HSPC, CD34 + CD38 + HPC, and CD34 + CD38 – CD90 + HSC to the VLA4 affinity detection ligand LDV-FITC. Data were normalized to LDV-FITC expression in CD34 + HSPC. Symbols represent unique MPB donors; n = 3 per group; statistical analysis was performed using 1-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001. In B – E and G , data were normalized to the indicated group, to account for variability in MFI between different experiments/donors.

Article Snippet: CD34 + CD38 – cells were isolated from G-CSF MPB by magnetic selection with human CD34 + CD38 – Isolation Kit (Miltenyi Biotec, 130-114-822) and cryopreserved.

Techniques: Injection, Irradiation, Expressing, MANN-WHITNEY, Binding Assay

Journal: JCI Insight

Article Title: Differential CXCR4 expression on hematopoietic progenitor cells versus stem cells directs homing and engraftment

doi: 10.1172/jci.insight.151847

Figure Lengend Snippet: ( A – C ) High CXCR4 expression on HPC mediates their preferential homing locally with i.b.m. transplant; CD34 + HSPC were transduced with a BFP LV vector, and the CD34 + CD38 + BFP + HPC were sorted by flow cytometry 72 hours after gene transfer. HPC were blocked with AMD3100 (CXCR4 antagonist) or BIO5192 (VLA4 antagonist) before i.v. or i.b.m. delivery ( A ). Homing of hCD34 + cells was analyzed in the injected femur (IF) and non-IF for i.b.m. injected mice and in both femurs combined in i.v. injected mice. Data were normalized to i.v. injected control CD34 + CD38 + cells, and the fold increase is indicated within the bar ( B and C ). n = 5–6 mice per group; statistical analysis was performed by 1-way ANOVA. ( D – F ) Induction of high CXCR4 expression on CD34 + CD38 – HSC enriched population via gene transfer confers them with a homing advantage despite higher abundance of CD34 + CD38 + HPC. CD34 + HSPC were transduced with a BFP LV vector or a BFP-CXCR4 LV vector and sorted for transduced CD34 + CD38 – BFP + cells and untransduced CD34 + CD38 + BFP – HPC at 72 hours. Transduced CD34 + CD38 – cells were mixed with untransduced CD34 + CD38 + cells and transplanted i.v. or i.b.m., and homing of CD34 + CD38 – BFP + cells into BM was analyzed at 20–22 hours ( D ). CXCR4 expression on injected cells at time of transplant is shown and the MFI on injected cells at time of transplant was as follows: CD34 + CD38 – control, 16,930; CD34 + CD38 + control, 35,098; and CD34 + CD38 – CXCR4 transduced, 37,453. ( E ). Homing in BM is shown ( F ). n = 3–10 mice per experimental arm; statistical analysis was performed using 1-way ANOVA. * P < 0.05.

Article Snippet: CD34 + CD38 – cells were isolated from G-CSF MPB by magnetic selection with human CD34 + CD38 – Isolation Kit (Miltenyi Biotec, 130-114-822) and cryopreserved.

Techniques: Expressing, Transduction, Plasmid Preparation, Flow Cytometry, Injection, Control

Journal: JCI Insight

Article Title: Differential CXCR4 expression on hematopoietic progenitor cells versus stem cells directs homing and engraftment

doi: 10.1172/jci.insight.151847

Figure Lengend Snippet: CD34 + CD38 – HSC-enriched cells were obtained using immunomagnetic sorting, transduced with a GFP LV vector (gene transfer efficiency was 69%), and transplanted into NSG mice i.v. or i.b.m. in 2 limiting dilution doses. ( A and B ) Long-term (24 week) engraftment of total (hCD45 + ) and GM human (hCD45 + GFP + ) cells is shown. Symbols represent individual animals; n = 3–4 mice per treatment condition; statistical analysis was performed by comparing the modes of delivery comparing the different cell doses using 1-way ANOVA. * P < 0.05. ( C and D ) Lineage output at 24 weeks shows that the long-term human graft was multilineage, composed of B, T, and myeloid cells and CD34 + HSPC. Statistical analysis was performed using 2-way ANOVA.

Article Snippet: CD34 + CD38 – cells were isolated from G-CSF MPB by magnetic selection with human CD34 + CD38 – Isolation Kit (Miltenyi Biotec, 130-114-822) and cryopreserved.

Techniques: Transduction, Plasmid Preparation

Journal: JCI Insight

Article Title: Differential CXCR4 expression on hematopoietic progenitor cells versus stem cells directs homing and engraftment

doi: 10.1172/jci.insight.151847

Figure Lengend Snippet: ( A ) Construct design. The aa sequence of VPR and its mutant version generated is shown. VPR, a small HIV-1 accessory protein, is carried in viral particles bound to gag via residues in its central region that fold into 3 α-helices. The C-terminal domain has 6 arginine residues that potentiate nuclear localization, G 2 M arrest, and apoptosis. S79 phosphorylation is important for cell cycle arrest. We truncated VPR at the 78 aa and mutated VPR R77Q . W54 and Q65 interacts with DNA damage response (DDR) proteins via UNG2 and DCAF and were mutated to VPR W54R and VPR Q65R . The triple mutated and truncated VPR (VPR MT ) would lack pathogenicity (residues known to be associated with VPR toxicity are highlighted in red) but allow binding to gag. VPR MT was fused to CXCR4 cDNA via the HIV-1 protease cleavage site (PCS) to generate VPR MT -CXCR4. ( B ) K562 cells were transduced with LV CXCR4 vector-like particles (VLP; empty vector particles lacking the vector genome) at increasing particle concentration and analyzed for CXCR4 expression using flow cytometry. Mean fluorescence intensity (MFI) of CXCR4 is listed against volume of VLP added. ( C and D ) A GFP-encoding LV was either packaged using standard packaging plasmids (LV, black) or packaged with VPR MT -CXCR4 plasmid in addition in order to package the CXCR protein attached to the LV capsid (LV CXCR4 , red). CXCR4 expression on MPB CD34 + cells transduced with LV CXCR4 vector compared with cells transduced with the control LV vector 24 hours following gene transfer is shown. n = 4; statistical analysis was performed by Mann-Whitney U test. ( E ) The time course of CXCR4 expression in LV CXCR4 HSPC, normalized to that of control LV HSPC, is shown, with expression peaking at 24 hours that returns to baseline by 72 hours. Symbols represent individual MPB donors; n = 3.

Article Snippet: CD34 + CD38 – cells were isolated from G-CSF MPB by magnetic selection with human CD34 + CD38 – Isolation Kit (Miltenyi Biotec, 130-114-822) and cryopreserved.

Techniques: Construct, Sequencing, Mutagenesis, Generated, Phospho-proteomics, Binding Assay, Transduction, Plasmid Preparation, Concentration Assay, Expressing, Flow Cytometry, Fluorescence, Control, MANN-WHITNEY

Journal: JCI Insight

Article Title: Differential CXCR4 expression on hematopoietic progenitor cells versus stem cells directs homing and engraftment

doi: 10.1172/jci.insight.151847

Figure Lengend Snippet: Experimental schema for assessment of homing and engraftment of CD34 + CD38 – cells transduced with GFP LV or GFP LV CXCR4 that were transplanted into NSG mice either via i.v. or i.b.m. delivery. ( A ) The number of animals used for the homing experiment and engraftment experiments is indicated under each experimental arm. ( B ) Homing of CD34 + CD38 – cells in the BM. Data were normalized to i.v. injected control CD34 + CD38 – cells, and the fold increase is indicated within the bar. Symbols represent individual mice; statistical analysis was performed using ANOVA. ( C and D ) Long-term engraftment of CD34 + CD38 – cells was assessed by determining the percentage of human CD45 + GFP – cells ( C ) and human CD45 + GFP + ( D ) cells 24 weeks following transplant. Data were normalized to i.v. transplanted GFP LV transduced CD34 + CD38 – cells, and the fold increase is indicated within the bar. Gene transfer was 60% with GFP LV and 52% with GFP LV CXCR4 . Symbols represent individual mice; statistical analysis was performed by 1-way ANOVA. * P < 0.05, ** P < 0.01.

Article Snippet: CD34 + CD38 – cells were isolated from G-CSF MPB by magnetic selection with human CD34 + CD38 – Isolation Kit (Miltenyi Biotec, 130-114-822) and cryopreserved.

Techniques: Transduction, Injection, Control

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 1-month-old goat testis. ( A-C ) DIF of CD34/α-SMA in 1-month-old goat testis; ( D-F ) DIF of CD34/α-SMA in blood vessels of 1-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); CD34 + cells (white triangular arrows); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-C ):10 μm; ( D-F ): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 2-month-old goat testis. ( A-C ) DIF of CD34/α-SMA in 2-month-old goat testis; ( D-F ) DIF of CD34/α-SMA in blood vessels of 2-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); CD34 + cells (white triangular arrows); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-C ): 20 μm; ( D-F ): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 12-month-old goat testis. ( A-I ) DIF of CD34/α-SMA in 12-month-old goat testis; ( J-L ) DIF of CD34/α-SMA in blood vessels of 12-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-F, J-L ): 10 μm; ( G-I ): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 1-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 1-month-old goat testis. (1-a), (1-b), (1-c) are respectively enlarged images of (1) in ( A ), ( B ), ( C ). (2-a), (2-b) and (2-c) are the enlarged images of (2) in ( A ), ( B ) and ( C ) respectively. CD34 (red); α-SMA (green); DAPI (blue); TC (yellow, indicated by white triangular arrows); ST. Seminiferous tubule; SC. Sertoli cells. Scale bar = ( A, B, C ): 20 μm; (1 - a) - (1 - c), (2- a) - (2 - c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 2-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 2-month-old goat testis. (a-c) are enlarged views of the boxed areas in ( A-C ). CD34 (red); α-SMA (green); DAPI (blue); TC (yellow, indicated by white triangular arrows); ST. Seminiferous tubule. Scale bar = ( A-C ): 20 μm; (a-c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. CD34 (red); Vimentin (green); DAPI (blue); ST. Seminiferous tubule; S. sperm; PS. Primary spermatocyte; SC. Sertoli cells; RS. Round sperm cells; White triangular arrows. Peritubular myoid cell nucleus. Scale bar = ( A-C ): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. (a-c) is an enlarged view of the white dotted box area of ( A-C ). CD34 (red); Vimentin (green); DAPI (blue); TC (yellow, indicated by white triangular arrows). Scale bar = ( A-C ), (a-c): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. (1-a), (1-b), (1-c) are respectively enlarged images of (1) in ( A-C ). (2-a), (2-b), (2-c) are the enlarged images of (2) in ( A-C ). CD34 (red); Vimentin (green); DAPI (blue); TC (yellow); White triangular arrows. Peritubular myoid cell nucleus. Scale bar = ( A-C ): 50 μm; (1-a) - (1-c), (2-a) - (2-c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Nucleic acids research

Article Title: A conserved nutrient responsive axis mediates autophagic degradation of miRNA-mRNA hybrids in blood cell progenitors.

doi: 10.1093/nar/gkad1047

Figure Lengend Snippet: Figure 1. Targeting miRNAs and amino acid sensing in Drosophila blood cell progenitors. ( A–A’ ) The primary lobe of the lymph gland was isolated from a 48 h AEH larvae by laser capture micro-dissection ( LCM ) technique. ( B ) Marker analysis was done after cDNA conversion of RNA isolates post-LCM dissection of the primary lobes, which is then visualized through Agarose gel electrophoresis. ( C ) Heatmap depicting the expression profile of the Drosophila miRNAs present in the primary lobe ( 48 h AEH ) retrie v ed through LCM. ( D–F’ ) EdU incorporation analysis of lymph glands at ( D, D’ ) 36 h, ( E, E’ ) 48 h and ( F, F’ ) 60 h upon Dicer1 down regulation from the progenitor. ( G–G’ ) Volumetric comparison by 3D-reconstruction of progenitors in the wildtype ( G ) and ( G’ ) Dcr1 knockdown lymph gland. ( H–K ) Quantitative time-kinetic analysis of W ildt ype and Dcr1KD progenitors depicting the ( H ) proliferation rate, ( I ) progenitor number, ( J ) progenitor volume and ( K ) individual progenitor cell volume observed at 36, 48 and 60 h AEH respectively. ( L–N’ ) Quantitative analysis of progenitor number and volume upon increasing concentration of Carbon sources. ( L –L ’ ) in e x cess Carboh y drates, ( M–M’ ) in e x cess lipids, ( N–N’ ) in e x cess proteins. Scale, 20 μm in all images. Individual dots represent ‘ n ’ of the sample. Significance w as e v aluated using tw o-w a y ANO V A with Tuk e y’s test w as perf ormed f or grouped analy ses. Error bar: standard de viation ( SD ) . Data are mean ± SD. * P < 0.033, ** P < 0.002 and *** P < 0.001. See also Supplementary Figure S1.

Article Snippet: Cell culture of human HSPC Primary Bone Marrow CD34+ Hematopoietic Cells, Normal, Human were acquired from ATCC ( PCS-800-012 ) .

Techniques: Isolation, Dissection, Marker, Agarose Gel Electrophoresis, Expressing, Comparison, Knockdown, Concentration Assay

Journal: Nucleic acids research

Article Title: A conserved nutrient responsive axis mediates autophagic degradation of miRNA-mRNA hybrids in blood cell progenitors.

doi: 10.1093/nar/gkad1047

Figure Lengend Snippet: Figure 5. Human blood progenitors exhibit leucine responsive proliferation similar to Drosophila . ( A–A’ ) EdU incorporation analysis of CD34+ CD10-Lin- m y eloid biased HSPC in undeprived and leucine-deficient media. ( B and B’ ) Quantitative analysis in the proliferation rate of CD34+ CD10-Lin- HSPCs and KG1 cells. ( C–D’ ) Dose-dependent reco v ery in the proliferation rate of CD34+ CD10-Lin– HSPC upon increasing concentration of leucine in leucine deprived media. ( D–D’ ) Quantitative analysis of the recovery in proliferation index of CD34+ CD10-Lin- HSPCs and KG1 cells. ( E ) OPP incorporation rate analysis of CD34+ CD10-Lin-myeloid biased HSPC in early stages ( 6 h ) of leucine deprivation versus late stages ( 16 h ) . ( F ) Ly sotrack er staining of acidic autolysosome upon leucine starvation at an early time point in HSPCs. ( G ) Cyto-ID staining of autophagosomes in response to leucine starvation at early time point in HSPCs. ( H ) Quantitativ e analy sis of OPP incorporation rate at 6 and 16 h of leucine starv ation condition in HSPCs. ( I ) Quantitative measurement of increased acidic vesicles ( Lysotracker ) in HSPCs. ( J ) Quantitative measurement of autophagosomes ( Cyto-ID ) in HSPCs. Scale, 20 μm in all images. Individual dots represent ‘n’ of the sample. Tw o-w a y ANOVA was performed for grouped analyses. One-way ANO V A was performed for individual multiple comparisons. Error bar: standard deviation ( SD ) . Data are mean ± SD. * P < 0.033, ** P < 0.002 and *** P < 0.001. See also Supplementary Figure S5.

Article Snippet: Cell culture of human HSPC Primary Bone Marrow CD34+ Hematopoietic Cells, Normal, Human were acquired from ATCC ( PCS-800-012 ) .

Techniques: Concentration Assay, Staining, Standard Deviation

Journal: Nucleic acids research

Article Title: A conserved nutrient responsive axis mediates autophagic degradation of miRNA-mRNA hybrids in blood cell progenitors.

doi: 10.1093/nar/gkad1047

Figure Lengend Snippet: Figure 6. Leucine-based proliferation of human blood progenitors also targets autophagy-dependent miRNA turnover. ( A ) Heatmap representing high throughput transcriptomic analysis at 6 h pulse of leucine starvation in CD34+ CD10-Lin– HPSCs. ( B ) Network map depicting pathway characterization of the differentially expressing genes in CD34+ CD10-Lin– HSPC as a response to leucine deprivation. ( B’ ) GO analysis of the most significantly affected biological process in CD34+ CD10-Lin- HSPCs as a response to leucine deprivation. ( C ) Western blot depicting reduction in Ago2 protein upon early and late response to leucine starvation. ( D ) Levels of p62 / SQSTM1 show a similar reduction as in ( C ) . ( E ) Co-immunoprecipitation depicting ph y sical interaction between p62 and Ago2. The blot depicts a basal level of Ago2 interaction that increases upon leucine deprivation ( Low exposure of the same blot is in Supplementary Figure S6G ) . ( F ) EdU incorporation analysis upon leucine starvation and simultaneous treatment with B afilom y cinA1 ( B afA1 ) . ( F’ ) Quantitative analysis on the proliferation rate upon leucine deprivation and BafA1 treatment. ( G ) Levels of Ago-2 upon leucine deprivation and BafA1 treatment detected by western blotting. ( H ) Quantitativ e analy sis of A go2 protein le v els upon leucine depriv ation and B afA1 treatment. ( I ) Schematic representing miRNA-9 sponge construct used for RNA-IP ( abo v e ) . Destabiliz ed GFP transcript with deleted sponge sites ( served as Control-Sponge ) and destabilized GFP transcript with 8x sponge sites ( miRNA-9 Sponge ) ( below ) . ( J ) Agarose gel demonstrates a qualitative assessment of miRNA-9 sponge enrichment upon p62-RNA-IP. ( J’ ) A qPCR-based analysis of Control-sponge and miRNA-9 sponge enrichment upon p62-RNA-IP and assessment of an endogenous transcript ( actin ) within the pulled fraction. ( K ) qPCR analysis of RNA-IP done with p62 as bait using Control-sponge and miRNA-9 sponge construct upon leucine deprivation ( left ) . qPCR analysis of puromycin transcript acts as a negative control ( right ) . ( K’ ) qPCR analysis of RNA-IP done with p62 on miRNA-9 sponge construct upon leucine deprivation and BafA1 treatment during leucine deprivation ( left ) . A qPCR analysis of GFP in the Input fraction of the same ( right ) . Scale, 20 μm in all images. Individual dots represent ‘n’ of the sample. Multiple t -test with Welch correction was performed for RNA-IP analysis. One-way ANO V A was performed for individual multiple comparisons. Error bar: standard deviation ( SD ) . Data are mean ± SD. * P < 0.033, ** P < 0.002 and *** P < 0.001.

Article Snippet: Cell culture of human HSPC Primary Bone Marrow CD34+ Hematopoietic Cells, Normal, Human were acquired from ATCC ( PCS-800-012 ) .

Techniques: High Throughput Screening Assay, Expressing, Western Blot, Immunoprecipitation, Construct, Control, Agarose Gel Electrophoresis, Negative Control, Standard Deviation

Journal: Scientific Reports

Article Title: New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

doi: 10.1038/s41598-021-89616-3

Figure Lengend Snippet: Immunohistochemical staining images showing CD34 expression in ( A ) normal mouse brain tissue and ( B – F ) mouse brain tissue on days 3, 7, 15, 21 and 30 days, following the pTBI. ( A ) CD34 was not expressed in normal mouse brain tissue. CD34-positive cells and blood vessels (brown; black arrows) became visible at the injury site (red arrow) on days ( B ) 3 and ( C ) 7. ( D ) After 15 days, CD34 expression at the injury site (red arrow) decreased. ( E ) CD34 expression at the injury site could not be detected on day 21. Instead, black hemosiderin particles (black arrows) were observed at the injury site. ( F ) Some black hemosiderin particles remained visible at the injury site on day 30. ( G ) Immunohistochemical results of CD34 were analyzed by Image-Pro Plus 6.0 software. The average density (IOD) of CD34-positive cells in normal mouse brain tissue and in mouse brain tissue on days 3, 7, 15, 21 and 30 days, following pTBI. The n = 10/group was used for calculating the average IOD. Comparisons between groups were performed using the Kruskal–Wallis H test followed by Bonferroni post-hoc analysis.

Article Snippet: The sections were first incubated with rabbit anti-mouse PROX1 antibody (1:100; Boster Biological Technology Co., Wuhan, China), rabbit anti-mouse CD34 antibody (1:100; BosterBiological Technology) or goat anti-mouse LYVE-1 antibody (1:100; R&D Systems, Shanghai, China) at 4 °C for 24 h. Next, the sections were incubated with horseradish peroxidase-labeled goat anti-rabbit IgG or rabbit anti-goat IgG secondary antibodies.

Techniques: Immunohistochemical staining, Staining, Expressing, Software

Journal: Scientific Reports

Article Title: New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

doi: 10.1038/s41598-021-89616-3

Figure Lengend Snippet: Double immunofluorescence staining images showing CD34/LYVE-1 expression in the brain tissue 3 days after pTBI. Expression of ( A ) LYVE-1 (green); ( B ) CD34 (red); and ( C ) merge (yellow).

Article Snippet: The sections were first incubated with rabbit anti-mouse PROX1 antibody (1:100; Boster Biological Technology Co., Wuhan, China), rabbit anti-mouse CD34 antibody (1:100; BosterBiological Technology) or goat anti-mouse LYVE-1 antibody (1:100; R&D Systems, Shanghai, China) at 4 °C for 24 h. Next, the sections were incubated with horseradish peroxidase-labeled goat anti-rabbit IgG or rabbit anti-goat IgG secondary antibodies.

Techniques: Double Immunofluorescence Staining, Expressing